姓 名：李涛 教授 电 话：0551-63601813 电子邮件：tlitao@ustc.edu.cn 传 真：0551-63601592 |  |
个人简历
2015.7月至今，中国科技大学化学系，教授
2014.5－2015.6，美国亚利桑那州立大学，博士后
2011.2－2014.4，德国波恩大学，洪堡学者/博士后
2007.9－2011.1，中科院长春应用化学研究所，博士
2004.7－2007.8，中科院长春应用化学研究所，助理研究员
2001.9－2004.6，武汉大学，硕士
1997.9－2001.6，山西大学，学士
主要研究方向
1. 四链核酸结构与功能研究及分析应用：设计开发折叠可控的双分子G-四链体与i-motif结构，用于DNA纳米组装及可开关传感分析；研究四链核酸与荧光配体作用情况，发展新型信号读出体系，用于免标记生物分析及细胞成像；研究四链核酸引导金纳米、金棒组装与等离子体增强情况，拓展其活体应用前景。
2. DNA纳米机器设计与应用：以DNA四面体等框架核酸为纳米脚手架，利用G-四链体、i-motif、ATP适配体等响应外界刺激，发展细胞微环境识别的智能型DNA逻辑电路；将DNA电路与纳米机器进行有机整合，在细胞、动物活体内原位控制分子载荷靶向传递与精准释放，进行microRNA、mRNA成像，抑制疾病基因表达。
3. 球形核酸酶(SNAzyme)催化的电化学发光及疾病检测应用：基于G-四链体与血红素在金纳米核表面组装，制备高活性、高稳定性的球形核酸酶，结合核酸放大技术，将球形核酸酶催化的电化学发光体系用于疾病相关核酸标志物灵敏检测；利用框架核酸搭建电化学发光纳米平台，对循环microRNA、外泌体RNA进行增强检测。
获奖及荣誉
2012年中科院优秀博士论文
2011年德国洪堡奖学金
文章及专著
1. Huihui Wang, Pai Peng, Qiwei Wang, Yi Du, Zhijin Tian, Tao Li*. Environment-Recognizing DNA-Computation Circuits for the Intracellular Transport of Molecular Payloads for mRNA Imaging. Angew. Chem. Int. Ed. 2020, 59, 6099–6107.
2. Lili Shi, Pai Peng, Jiao Zheng, Qiwei Wang, Zhijin Tian, Huihui Wang, Tao Li*. I-Motif/miniduplex hybrid structures bind benzothiazole dyes with unprecedented efficiencies: a generic light-up system for label-free DNA nanoassemblies and bioimaging. Nucleic Acids Res. 2020, 48, 1681–1690.
3. Pai Peng, Yi Du, Jiao Zheng, Huihui Wang, Tao Li*. Reconfigurable Bioinspired Framework Nucleic Acid Nanoplatform Dynamically Manipulated in Living Cells for Subcellular Imaging. Angew. Chem. Int. Ed. 2019, 58, 1648-1653.
4. Jiao Zheng, Yi Du, Huihui Wang, Pai Peng, Lili Shi, Tao Li*. Ultrastable Bimolecular G-Quadruplexes Programmed DNA Nanoassemblies for Reconfigurable Biomimetic DNAzymes. ACS Nano 2019, 13, 11947-11954.
5. Yi Du, Pai Peng, Tao Li*. DNA Logic Operations in Living Cells Utilizing Lysosome-Recognizing Framework Nucleic Acid Nanodevices for Subcellular Imaging. ACS Nano 2019, 13, 5778-5784.
6. Yudie Sun, Qiwei Wang, Lan Mi, Lin Shi, Tao Li*. Target-Induced Payload Amplification for Spherical Nucleic Acid Enzyme (SNAzyme) Catalyzed Electrochemiluminescence Detection of Circulating miRNAs. Anal. Chem. 2019, 91, 12948-12953.
7. Yudie Sun, Lin Shi, Qiwei Wang, Lan Mi, Tao Li*. Spherical Nucleic Acid Enzyme (SNAzyme) Boosted Chemiluminescence miRNA Imaging Using a Smartphone. Anal. Chem. 2019, 91, 3652-3658.
8. Yudie Sun, Tao Li*. Composition-Tunable Hollow Au/Ag SERS Nanoprobes Coupled with Target-Catalyzed Hairpin Assembly for Triple-Amplification Detection of miRNA. Anal. Chem. 2018, 90, 11614-11621.
9. Lili Shi, Pai Peng, Yi Du, Tao Li*. Programmable i-motif DNA folding topology for a pH-switched reversible molecular sensing device. Nucleic Acids Res. 2017, 45, 4306-4314.
10. Shuangna Liu, Pai Peng, Huihui Wang, Lili Shi*, Tao Li*. Thioflavin T binds dimeric parallel-stranded GA-containing non-G-quadruplex DNAs: a general approach to lighting up double-stranded scaffolds. Nucleic Acids Res. 2017, 45, 12080–12089.
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